UAF, a yeast RNA polymerase I transcription factor, contains Rrn5p, Rrn9p, Rrn10p, histones H3 and H4, and uncharacterized protein p30. Mutants defective in RRN5, RRN9 or RRN10 are unable to transcribe rDNA by polymerase I and grow extremely slowly, but give rise to variants able to grow by transcribing chromosomal rDNA by polymerase II. Thus, UAF functions as both an activator of polymerase I and a silencer of polymerase II for rDNA transcription. We have now identified the gene for subunit p30. This gene, UAF30, is not essential for growth, but its deletion decreases the cellular growth rate. Remarkably, the deletion mutants use both polymerase I and II for rDNA transcription, indicating that the silencer function of UAF is impaired, even though rDNA transcription by polymerase I is still occurring. A UAF complex isolated from the uaf30 deletion mutant was found to retain the in vitro polymerase I activator function to a large extent. Thus, Uaf30p plays only a minor role in its activator function. Possible reasons for slow growth caused by uaf30 mutations are discussed.
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Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
---|---|---|---|---|---|---|
RNA polymerase I upstream activating factor complex | involved in | positive regulation of transcription by RNA polymerase I | IDA | ComplexPortal | 2014-10-16 | |
RNA polymerase I upstream activating factor complex | involved in | nucleolar large rRNA transcription by RNA polymerase I | IDA | ComplexPortal | 2014-10-16 | |
RNA polymerase I upstream activating factor complex | enables | RNA polymerase I cis-regulatory region sequence-specific DNA binding | IPI | ComplexPortal | 2014-10-16 | |
RNA polymerase I upstream activating factor complex | located in | nucleus | BSR | ComplexPortal | 2014-10-16 | |
UAF30 | part of | RNA polymerase I upstream activating factor complex | IDA | SGD | 2013-08-07 | |
UAF30 | involved in | nucleolar large rRNA transcription by RNA polymerase I | IMP | SGD | 2013-08-07 |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.
Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Interactor | Interactor | Assay | Annotation | Action | Modification | |
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HHF1 | RRN5 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification | |
HHT1 | RRN5 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification | |
RRN10 | RRN5 | Affinity Capture-MS | manually curated | Hit-Bait | No Modification | |
RRN5 | RRN9 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification | |
RRN5 | HHF2 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification | |
RRN5 | HHT2 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification | |
RRN5 | UAF30 | Affinity Capture-MS | manually curated | Bait-Hit | No Modification |