Reference: Sato M, et al. (2001) Yeast Saccharomyces cerevisiae has two cis-prenyltransferases with different properties and localizations. Implication for their distinct physiological roles in dolichol synthesis. Genes Cells 6(6):495-506

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Abstract


Background: Dolichol is a family of long-chain polyprenols, which is utilized as a sugar carrier in protein glycosylation in the endoplasmic reticulum (ER). We have identified a key enzyme of the dolichol synthesis, cis-prenyltransferase, as Rer2p from Saccharomyces cerevisiae. We have also isolated a multicopy suppressor of an rer2 mutant and named it SRT1. It encodes a protein similar to Rer2p but its function has not been established.

Results: The cis-prenyltransferase activity of Srt1p has been proved biochemically in the lysate of yeast cells lacking Rer2p. The polyprenol product of Srt1p is longer in chain length than that of Rer2p and is not sufficiently converted to dolichol and dolichyl phosphate, unlike that of Rer2p. The subcellular localization of these two isozymes has been examined by immunofluorescence microscopy and by the use of GFP fusion proteins. Whereas GFP-Rer2p is localized to the continuous ER and some dots associated with the ER, GFP-Srt1p shows only punctate localization patterns. Immunofluorescence double staining with Erg6p, a marker of lipid particles in yeast, indicates that Srt1p is mainly localized to lipid particles (lipid bodies). RER2 is mainly expressed in the early logarithmic phase, while the expression of SRT1 is induced in the stationary phase.

Conclusions: We have shown that yeast has two active cis-prenyltransferases with different properties. This result implies that the two isozymes have different physiological roles during the life cycle of the yeast.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Sato M, Fujisaki S, Sato K, Nishimura Y, Nakano A
Primary Lit For
RER2 | SRT1 | Dehydrodolichyl diphosphate synthase complex variant RER2 | Dehydrodolichyl diphosphate synthase complex variant SRT1
Additional Lit For
ERG6

Gene Ontology Annotations 6 entries for 2 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
RER2located inendoplasmic reticulumIDASGD2013-08-07
RER2enablesditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activityIDASGD2013-08-07
SRT1involved inprotein glycosylationIDASGD2013-08-07
SRT1enablesditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activityIDASGD2013-08-07
SRT1involved inprotein glycosylationIMPSGD2013-08-07
SRT1located inlipid dropletIDASGD2013-08-07
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Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 0 entries for 0 genes

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InteractorInteractorAssayAnnotationActionModification
No physical interaction data for Sato M, et al. (2001)
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