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Reference: Blasco A and Sanz P (2000) Disruption and functional analysis of six ORFs on chromosome IV: YDL053c, YDL072c, YDL073w, YDL076c, YDL077c and YDL080c. Yeast 16(15):1437-43

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Abstract

Six open reading frames (ORFs) from chromosome IV, YDL053c, YDL072c, YDL073w, YDL076c, YDL077c and YDL080c, were disrupted using the long flanking homology technique (LFH) to replace each target locus with the KanMX4 selection marker. We have also constructed plasmids containing replacement cassettes (pYORC) and the cognate clones (pYCG) for each ORF. Disruption of five of the ORFs-YDL053c, YDL072c, YDL073w, YDL076c and YDL080c (THI3)-resulted in no distinctive phenotype with respect to temperature or nutritional requirements. However, disruption of YDL077c (also known as VAM6) exhibited an slow growth phenotype in minimal media and also in rich media containing glycerol as a carbon source. The homozygous disruptant diploid corresponding to this gene also failed to sporulate.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Blasco A, Sanz P
Primary Lit For
RXT3
Additional Lit For
AHK1 | VAM6 | THI3 | PBP4 | YET3

Phenotype Annotations 1 entry for 1 gene

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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
RXT3viable
classical geneticsnull
Allele: rxt3-Δ		CEN.PKDetails: null also viable in FY1679 background
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