In the post-genome sequencing era the functional analysis of newly discovered proteins becomes more and more important. In this report we describe a genetic approach to the post-translational regulation of protein function in Saccharomyces cerevisiae by creating conditional lethal mutants. The yeast ORFs YDL139c, YDL147w, ERG3 and ERG11 were tagged with sequences encoding the hormone-binding domains of mammalian steroid receptors by PCR-mediated, targeted integration into the yeast genome. We found that the function of the chimeric proteins is regulated in a hormone-dependent way. This technique provides another important tool for the functional analysis of the yeast proteome.

• PMID: 11085272
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Reference Type

Journal Article | Research Support, Non-U.S. Gov't

Authors

Launhardt H, Munder T

Primary Lit For

SCM3 | ERG11 | RPN5 | ERG3

Additional Lit For

Review For