Reference: Rodriguez K, et al. (1996)
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Abstract
Saccharomyces cerevisiae rad1 and rad10 mutants are unable to carry out nucleotide excision repair and are also defective in a mitotic intrachromosomal recombination pathway. The products of these genes are subunits of an endonuclease which recognizes DNA duplex/single-strand junctions and specifically cleaves the 3' single-strand extension at or near the junction. It has been suggested that such junctions arise as a consequence of DNA lesion processing during nucleotide excision repair and the processing of double-strand breaks during intrachromosomal recombination. In this study we show that the RAD1 RAD10 complex also cleaves a more complex junction structure consisting of a duplex with a protruding 3' single-strand branch that resembles putative recombination intermediates in the RAD1 RAD10-mediated single-strand annealing pathway of mitotic recombination. Using monoclonal antibodies, we have identified two regions of RAD1 that are required for the cleavage of duplex/single-strand junctions. These reagents also inhibit nucleotide excision repair in vitro, confirming the essential role of the RAD1 RAD10 endonuclease in this pathway.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, P.H.S.
- Authors
-
Rodriguez K,
Wang Z,
Friedberg EC,
Tomkinson AE
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- RAD1
- RAD10 | SLX4-RAD1-RAD10 endonuclease complex | SAW1-RAD1-RAD10 endonuclease complex
Gene Ontology Annotations
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Evidence ID |
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Qualifier |
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
| RAD10 | RAD1 | Reconstituted Complex | manually curated | Hit-Bait | No Modification |
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