Reference: Lamping E, et al. (1994) Isolation and characterization of a mutant of Saccharomyces cerevisiae with pleiotropic deficiencies in transcriptional activation and repression. Genetics 137(1):55-65

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Abstract


The isolation of the dep1 mutant of Saccharomyces cerevisiae is reported. The mutant was identified by its disability to regulate expression of structural genes involved in phospholipid biosynthesis, INO1, CHO1 and OPI3, in response to supplementation with soluble lipid precursors. Expression of the INO1, CHO1 and OPI3 genes was not fully derepressed in the absence of soluble lipid precursors, inositol and choline in the dep1 mutant, as compared to wild type. The mutant also exhibited incomplete repression of these same genes in the presence of inositol and choline. Repression by phosphate of the PHO5 gene was reduced in the mutant, as was derepression of this gene in the absence of phosphate. In addition, we show that expression of INO1 and OPI3 structural genes is strongly dependent on the growth phase both in wild-type and dep1 mutant strains. However, in the mutant, elevated basal steady-state mRNA levels were reached in the late stationary growth phase, independent of supplementation conditions. The dep1 mutation represents a new complementation group with respect to phospholipid synthesis and was mapped to a position of about 12 cM distal from the centromere on the left arm of chromosome I. Deficiencies in transcription activation and repression of metabolically unrelated genes, as well as reduced mating efficiency and lack of sporulation of homozygous diploid dep1/dep1 mutants indicate a pleiotropic regulatory function of the DEP1 gene product. Thus, Dep1p appears to be a new member of a class of transcriptional modulators, including Rpd1p/Sin3p/Ume4p/Sdi1p/Gam3p, Rpd3p, Spt10p and Spt21p.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Lamping E, Lückl J, Paltauf F, Henry SA, Kohlwein SD
Primary Lit For
DEP1 | SIN3
Additional Lit For
PHO5 | INO1 | OPI3 | CHO1 | SPT10 | RPD3 | SPT21

Gene Ontology Annotations 13 entries for 1 gene


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
DEP1involved innegative regulation of transcription by RNA polymerase IIpart of negative regulation of inositol biosynthetic processIMPSGD2020-02-13
DEP1involved innegative regulation of transcription by RNA polymerase IIpart of negative regulation of phosphatidylserine biosynthetic processIMPSGD2020-02-13
DEP1involved innegative regulation of transcription by RNA polymerase IIpart of negative regulation of phosphatidylcholine biosynthetic processIMPSGD2020-02-13
DEP1involved innegative regulation of phosphatidylserine biosynthetic processIMPSGD2020-02-13
DEP1involved inpositive regulation of inositol biosynthetic processIMPSGD2019-08-30
DEP1involved innegative regulation of phosphatidylcholine biosynthetic processIMPSGD2020-02-13
DEP1involved innegative regulation of inositol biosynthetic processIMPSGD2019-08-30
DEP1involved inpositive regulation of phosphatidylcholine biosynthetic processIMPSGD2020-02-13
DEP1involved inpositive regulation of phosphatidylserine biosynthetic processIMPSGD2020-02-13
DEP1involved inregulation of transcription by RNA polymerase IIIMPSGD2013-08-07
Showing 1 to 10 of 13 entries

Phenotype Annotations 5 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
DEP1cell size: increased
classical geneticsunspecifiedW303Details: dep1 cells 40% larger in late stationary phase
DEP1mating efficiency: decreased
classical geneticsunspecifiedW303Details: dep1 cells mate ~10-fold less efficiently than wild-type after 7 hours incubation; independent of mutant mating type
DEP1RNA accumulation: increased
Reporter: INO1-lacZ mRNA

beta-galactosidase measured

classical geneticsunspecifiedW303Media: grown in presence of inositol and choline
DEP1sporulation: decreased
homozygous diploidunspecifiedW303Details: sporulation reduced to below 1% compared with wild-type
DEP1viability: decreased
classical geneticsunspecifiedW303Details: 50% reduction in viability at late stationary phase
Showing 1 to 5 of 5 entries