Broach JR, et al. (1979)

Abstract

We have constructed a plasmid, YEp13, which when used in conjunction with transformation in yeast is a suitable vector for isolating specific yeast genes. The plasmid consists of pBR322, the LEU2 gene of yeast, and a DNA fragment containing a yeast origin of replication from 2 mu circule. We have demonstrated the utility of this cloning system by isolating the yeast gene encoding the arginine permease, CAN1, from a pool of random yeast DNA fragments inserted into YEp13.

PMID: 395029
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Reference Type: Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors: Broach JR, Strathern JN, Hicks JB
Primary Lit For: CAN1
Additional Lit For: LEU2
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