Tetraploid yeast cells lacking BFR1 or overexpressing an essential gene BBP1 produce a novel type of ascus that contains asci instead of spores. We show here that the asci within an ascus likely arise because a/alpha spores undergo a second round of meiosis. Cells depleted of Bbp1p or lacking Bfr1p are defective in a number of processes such as nuclear segregation, bud formation, cytokinesis and nuclear spindle formation. Furthermore, deletion of BFR1 or overexpression of BBP1 leads to an increase in cell ploidy, indicating that Bfr1p and Bbp1p play roles in both the mitotic cell cycle and meiosis. Bfr1p and Bbp1p interact with each other in a two hybrid assay, further suggesting that they might form a complex important for cell cycle coordination.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| BFR1 | involved in | regulation of mitotic nuclear division | IMP | SGD | 2013-08-07 | |
| BFR1 | involved in | meiotic cell cycle | IMP | SGD | 2017-03-29 |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| BFR1 | cell cycle progression in anaphase: delayed | classical genetics | null Allele: bfr1-Δ | W303 | Details: subpopulation of large budded cells with a short spindle, inside the mother or bud neck | |
| BBP1 | cellular morphology: abnormal | classical genetics | repressible | W303 | Details: reduction in the percentage of small budded cells with many interconnected cells; higher percentage of cells with a 2N DNA content and some with a 4N DNA content | |
| BBP1 | chitin deposition: abnormal | classical genetics | repressible | W303 | Details: chitin is delocalized and found on the whole cell surface; bud scars and the bud neck chitin ring are difficult to detect | |
| BBP1 | inviable | classical genetics | overexpression | W303 | Details: overexpression at high levels (GALp) is toxic | |
| BBP1 | inviable | classical genetics | null Allele: bbp1-Δ | W303 | ||
| BBP1 | polyploidy: increased | classical genetics | overexpression | W303 | Details: overexpression at lower levels (ADHp) increases the proportion of cells with 4N and 8N DNA content | |
| BFR1 | polyploidy: increased | classical genetics | null Allele: bfr1-Δ | W303 | Details: culture contains both haploid (1N and 2N) as well as diploid (4N) cells | |
| BFR1 | polyploidy: increased | homozygous diploid | null Allele: bfr1-Δ | W303 | Details: culture contains both diploid (2N and 4N) as well as tetraploid (4N) cells | |
| BBP1 | spindle morphology: absent | classical genetics | repressible | W303 | Details: no nuclear spindles connecting SPBs are observed, in either large budded or unbudded cells | |
| BBP1 | sporulation: abnormal | homozygous diploid | overexpression | W303 | Details: overexpression at lower levels (ADHp) increases the percentage of tetraploid cells, and results in the formation of asci containing internal asci, possibly resulting from spores undergoing a second round of meiosis |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.