Reference: Helliwell SB, et al. (1994) TOR1 and TOR2 are structurally and functionally similar but not identical phosphatidylinositol kinase homologues in yeast. Mol Biol Cell 5(1):105-18

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Abstract


The Saccharomyces cerevisiae genes TOR1 and TOR2 were originally identified by mutations that confer resistance to the immunosuppressant rapamycin. TOR2 was previously shown to encode an essential 282-kDa phosphatidylinositol kinase (PI kinase) homologue. The TOR1 gene product is also a large (281 kDa) PI kinase homologue, with 67% identity to TOR2. TOR1 is not essential, but a TOR1 TOR2 double disruption uniquely confers a cell cycle (G1) arrest as does exposure to rapamycin; disruption of TOR2 alone is lethal but does not cause a cell cycle arrest. TOR1-TOR2 and TOR2-TOR1 hybrids indicate that carboxy-terminal domains of TOR1 and TOR2 containing a lipid kinase sequence motif are interchangeable and therefore functionally equivalent; the other portions of TOR1 and TOR2 are not interchangeable. The TOR1-1 and TOR2-1 mutations, which confer rapamycin resistance, alter the same potential protein kinase C site in the respective protein's lipid kinase domain. Thus, TOR1 and TOR2 are likely similar but not identical, rapamycin-sensitive PI kinases possibly regulated by phosphorylation. TOR1 and TOR2 may be components of a novel signal transduction pathway controlling progression through G1.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Helliwell SB, Wagner P, Kunz J, Deuter-Reinhard M, Henriquez R, Hall MN
Primary Lit For
TOR1 | TOR2
Additional Lit For
TOR1-1 | TOR2-1

Gene Ontology Annotations 2 entries for 2 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
TOR1involved inTOR signalingIMPSGD2013-08-07
TOR2involved inTOR signalingIMPSGD2013-08-07
Showing 1 to 2 of 2 entries

Phenotype Annotations 1 entry for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
TOR1vegetative growth: decreased
classical genetics null
Allele: TOR1-1

S1972R; phosphatidylinositol 3-kinase related domain mutated; recessive for rapamycin resistance at 24 deg C, and partially dominant at 30 deg C and 37 deg C

JK9-3dDetails: 10-15% increase in the generation time at 30 deg C; 15-25% increase at 24 deg C and 37 deg C
Showing 1 to 1 of 1 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 0 entries for 0 genes

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InteractorInteractorAssayAnnotationActionModification
No physical interaction data for Helliwell SB, et al. (1994)
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