A Candida albicans expression library was constructed from RNA isolated from regenerating protoplasts. A 1.4-kb cDNA clone was used to isolate a genomic fragment. Sequence analysis revealed an open reading frame of 593 amino acids with an overall identity of 63.6% with the phenylalanyl-tRNA synthetase beta subunit (FRS1) of Saccharomyces cerevisiae. We named it CaFRS1. It is located in a single copy in chromosome R, SfiI fragment M. Its expression showed a decrease during the cell wall regeneration process in protoplasts of both yeast and mycelial cells of C. albicans, suggesting its requirement thereof in initial steps of the cell wall synthesis.

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Reference Type

Journal Article | Research Support, Non-U.S. Gov't

Authors

Marcilla A, Pallotti C, Gomez-Lobo M, Caballero P, Valentin E, Sentandreu R

Primary Lit For

Phenylalanyl-tRNA synthetase complex

Additional Lit For

FRS1

Review For