Reference: Feldheim D, et al. (1993) Structural and functional characterization of Sec66p, a new subunit of the polypeptide translocation apparatus in the yeast endoplasmic reticulum. Mol Biol Cell 4(9):931-9

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Abstract


SEC66 encodes the 31.5-kDa glycoprotein of the Sec63p complex, an integral endoplasmic reticulum membrane protein complex required for translocation of presecretory proteins in Saccharomyces cerevisiae. DNA sequence analysis of SEC66 predicts a 23-kDa protein with no obvious NH2-terminal signal sequence but with one domain of sufficient length and hydrophobicity to span a lipid bilayer. Antibodies directed against a recombinant form of Sec66p were used to confirm the membrane location of Sec66p and that Sec66p is a glycoprotein of 31.5 kDa. A null mutation in SEC66 renders yeast cells temperature sensitive for growth. sec66 cells accumulate some secretory precursors at a permissive temperature and a variety of precursors at the restrictive temperature. sec66 cells show defects in Sec63p complex formation. Because sec66 cells affect the translocation of some, but not all secretory precursor polypeptides, the role of Sec66p may be to interact with the signal peptide of presecretory proteins.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Feldheim D, Yoshimura K, Admon A, Schekman R
Primary Lit For
SEC66
Additional Lit For
SEC63 | SEC62-SEC63 complex

Gene Ontology Annotations 2 entries for 1 gene


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
SEC66involved inpost-translational protein targeting to membrane, translocationhas input KAR2IMPSGD2019-11-06
SEC66part ofSec62/Sec63 complexIPI with SEC63SGD2013-08-07
Showing 1 to 2 of 2 entries

Phenotype Annotations 3 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
SEC66heat sensitivity: increased
classical geneticsnull
Allele: sec66-Δ
Other
SEC66protein/peptide accumulation: increased
Reporter: invertase precursor
classical geneticsnull
Allele: sec66-Δ
Other
SEC66protein/peptide accumulation: increased
Reporter: Kar2p precursor
classical geneticsnull
Allele: sec66-Δ
Other
Showing 1 to 3 of 3 entries