Reference: Mayes AE, et al. (1999) Characterization of Sm-like proteins in yeast and their association with U6 snRNA. EMBO J 18(15):4321-31

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Abstract


Seven Sm proteins associate with U1, U2, U4 and U5 spliceosomal snRNAs and influence snRNP biogenesis. Here we describe a novel set of Sm-like (Lsm) proteins in Saccharomyces cerevisiae that interact with each other and with U6 snRNA. Seven Lsm proteins co-immunoprecipitate with the previously characterized Lsm4p (Uss1p) and interact with each other in two-hybrid analyses. Free U6 and U4/U6 duplexed RNAs co-immunoprecipitate with seven of the Lsm proteins that are essential for the stable accumulation of U6 snRNA. Analyses of U4/U6 di-snRNPs and U4/U6.U5 tri-snRNPs in Lsm-depleted strains suggest that Lsm proteins may play a role in facilitating conformational rearrangements of the U6 snRNP in the association-dissociation cycle of spliceosome complexes. Thus, Lsm proteins form a complex that differs from the canonical Sm complex in its RNA association(s) and function. We discuss the possible existence and functions of alternative Lsm complexes, including the likelihood that they are involved in processes other than pre-mRNA splicing.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Mayes AE, Verdone L, Legrain P, Beggs JD
Primary Lit For
SNR6 | LSM8 | LSM2 | LSM6 | LSM1 | LSM4 | LSM5 | LSM7 | LSM3 | LSM1-7-PAT1 complex | U6 small nuclear ribonucleoprotein complex | ... Show all

Gene Ontology Annotations 7 entries for 7 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
LSM2involved inmRNA splicing, via spliceosomeIMPSGD2019-06-19
LSM6involved inmRNA splicing, via spliceosomeIMPSGD2019-06-19
LSM4involved inmRNA splicing, via spliceosomeIMPSGD2019-06-19
LSM5involved inmRNA splicing, via spliceosomeIMPSGD2019-06-19
LSM8involved inmRNA splicing, via spliceosomeIMPSGD2019-06-19
LSM3involved inmRNA splicing, via spliceosomeIMPSGD2019-06-19
LSM7involved inmRNA splicing, via spliceosomeIMPSGD2019-06-19
Showing 1 to 7 of 7 entries

Phenotype Annotations 13 entries for 7 genes


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
LSM1growth in exponential phase: decreased rate
classical geneticsnull
Allele: lsm1-Δ
OtherTemperature: permissive temperature, 23 °C
Details: slow growth rate at 23 and 30 deg C
LSM6growth in exponential phase: decreased rate
classical geneticsnull
Allele: lsm6-Δ
OtherTemperature: permissive temperature, 23 °C
Details: slow growth rate at 23 and 30 deg C
LSM7growth in exponential phase: decreased rate
classical geneticsnull
Allele: lsm7-Δ
OtherTemperature: permissive temperature, 23 °C
Details: slow growth rate at 23 and 30 deg C
LSM1heat sensitivity: increased
classical geneticsnull
Allele: lsm1-Δ
OtherTemperature: elevated temperature, 37 °C
LSM6heat sensitivity: increased
classical geneticsnull
Allele: lsm6-Δ
OtherTemperature: elevated temperature, 37 °C
LSM7heat sensitivity: increased
classical geneticsnull
Allele: lsm7-Δ
OtherTemperature: elevated temperature, 37 °C
LSM3inviable
classical geneticsnull
Allele: lsm3-Δ
Other
LSM5inviable
classical geneticsnull
Allele: lsm5-Δ
Other
LSM8inviable
classical geneticsnull
Allele: lsm8-Δ
Other
LSM2inviable
classical geneticsnull
Allele: lsm2-Δ
Other
Showing 1 to 10 of 13 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 63 entries for 10 genes

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InteractorInteractorAssayAnnotationActionModification
LSM1LSM1Two-hybridmanually curatedHit-BaitNo Modification
LSM1LSM3Two-hybridmanually curatedBait-HitNo Modification
LSM1LSM7Two-hybridmanually curatedBait-HitNo Modification
LSM1LSM7Two-hybridmanually curatedHit-BaitNo Modification
LSM1LSM8Two-hybridmanually curatedBait-HitNo Modification
LSM2LSM1Two-hybridmanually curatedHit-BaitNo Modification
LSM2LSM1Two-hybridmanually curatedBait-HitNo Modification
LSM2LSM3Two-hybridmanually curatedBait-HitNo Modification
LSM2LSM3Two-hybridmanually curatedHit-BaitNo Modification
LSM2LSM4Two-hybridmanually curatedBait-HitNo Modification
Showing 1 to 10 of 63 entries