Seven Sm proteins associate with U1, U2, U4 and U5 spliceosomal snRNAs and influence snRNP biogenesis. Here we describe a novel set of Sm-like (Lsm) proteins in Saccharomyces cerevisiae that interact with each other and with U6 snRNA. Seven Lsm proteins co-immunoprecipitate with the previously characterized Lsm4p (Uss1p) and interact with each other in two-hybrid analyses. Free U6 and U4/U6 duplexed RNAs co-immunoprecipitate with seven of the Lsm proteins that are essential for the stable accumulation of U6 snRNA. Analyses of U4/U6 di-snRNPs and U4/U6.U5 tri-snRNPs in Lsm-depleted strains suggest that Lsm proteins may play a role in facilitating conformational rearrangements of the U6 snRNP in the association-dissociation cycle of spliceosome complexes. Thus, Lsm proteins form a complex that differs from the canonical Sm complex in its RNA association(s) and function. We discuss the possible existence and functions of alternative Lsm complexes, including the likelihood that they are involved in processes other than pre-mRNA splicing.
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Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
---|---|---|---|---|---|---|
LSM2 | involved in | mRNA splicing, via spliceosome | IMP | SGD | 2019-06-19 | |
LSM6 | involved in | mRNA splicing, via spliceosome | IMP | SGD | 2019-06-19 | |
LSM4 | involved in | mRNA splicing, via spliceosome | IMP | SGD | 2019-06-19 | |
LSM5 | involved in | mRNA splicing, via spliceosome | IMP | SGD | 2019-06-19 | |
LSM8 | involved in | mRNA splicing, via spliceosome | IMP | SGD | 2019-06-19 | |
LSM3 | involved in | mRNA splicing, via spliceosome | IMP | SGD | 2019-06-19 | |
LSM7 | involved in | mRNA splicing, via spliceosome | IMP | SGD | 2019-06-19 |
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Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
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LSM1 | growth in exponential phase: decreased rate | classical genetics | null Allele: lsm1-Δ | Other | Temperature: permissive temperature, 23 °C Details: slow growth rate at 23 and 30 deg C | |
LSM6 | growth in exponential phase: decreased rate | classical genetics | null Allele: lsm6-Δ | Other | Temperature: permissive temperature, 23 °C Details: slow growth rate at 23 and 30 deg C | |
LSM7 | growth in exponential phase: decreased rate | classical genetics | null Allele: lsm7-Δ | Other | Temperature: permissive temperature, 23 °C Details: slow growth rate at 23 and 30 deg C | |
LSM1 | heat sensitivity: increased | classical genetics | null Allele: lsm1-Δ | Other | Temperature: elevated temperature, 37 °C | |
LSM6 | heat sensitivity: increased | classical genetics | null Allele: lsm6-Δ | Other | Temperature: elevated temperature, 37 °C | |
LSM7 | heat sensitivity: increased | classical genetics | null Allele: lsm7-Δ | Other | Temperature: elevated temperature, 37 °C | |
LSM3 | inviable | classical genetics | null Allele: lsm3-Δ | Other | ||
LSM5 | inviable | classical genetics | null Allele: lsm5-Δ | Other | ||
LSM8 | inviable | classical genetics | null Allele: lsm8-Δ | Other | ||
LSM2 | inviable | classical genetics | null Allele: lsm2-Δ | Other |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Interactor | Interactor | Assay | Annotation | Action | Modification | |
---|---|---|---|---|---|---|
LSM1 | LSM1 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
LSM1 | LSM3 | Two-hybrid | manually curated | Bait-Hit | No Modification | |
LSM1 | LSM7 | Two-hybrid | manually curated | Bait-Hit | No Modification | |
LSM1 | LSM7 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
LSM1 | LSM8 | Two-hybrid | manually curated | Bait-Hit | No Modification | |
LSM2 | LSM1 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
LSM2 | LSM1 | Two-hybrid | manually curated | Bait-Hit | No Modification | |
LSM2 | LSM3 | Two-hybrid | manually curated | Bait-Hit | No Modification | |
LSM2 | LSM3 | Two-hybrid | manually curated | Hit-Bait | No Modification | |
LSM2 | LSM4 | Two-hybrid | manually curated | Bait-Hit | No Modification |