Translation of mitochondrial cytochrome b in Saccharomyces cerevisiae requires the nuclearly encoded proteins Cbs1p, Cbs2p and Cbp6p. So far no homologs have been identified, except for the product of the S. cerevisiae orf YHR063C, which has some similarity to Cbs2p. Here we analyze the effect of a null mutation of YHR063C and show that it is not required for mitochondrial respiration. In addition, we report on the importance of the carboxyl-terminus of Cbs2p for its function. We show that truncations and some directed mutations in the carboxyl-terminal region of Cbs2p render the protein non-functional. The importance of the COOH-terminus is further underscored by the finding that mutational alteration of the cbs2-1 allele results in the substitution of Ile(372) by Lys.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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