A soluble cell-free extract containing RNA polymerase III and factors essential for selective transcription of the yeast SUP4-o tRNATyr gene was prepared from Saccharomyces cerevisiae cells. An intragenic promoter for yeast RNA polymerase III was identified within the yeast tRNATyr coding sequence by testing several sup4 genes with 5'- and 3'-terminal deletions in the homologous transcription system. Thirty-four different sup4 genes with spontaneous mutations were also tested in the in vitro system. Two point mutations drastically reduced transcription initiation and two other mutations caused premature termination. These mutations have nearly identical effects on SUP4 gene transcription by Xenopus RNA polymerase III (1), which demonstrates that the essential features of RNA polymerase III transcription initiation and termination signals have been conserved throughout the course of eukaryotic evolution.

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Reference Type

Journal Article | Research Support, U.S. Gov't, P.H.S.

Authors

Koski RA, Allison DS, Worthington M, Hall BD

Primary Lit For

DNA-directed RNA polymerase III complex

Additional Lit For

RPC17 | SUP4 | RPC25 | RPC10 | RPB5 | RPC37 | RPC53 | RPC11 | RPC40 | RPC19 | RPO31 | ... Show allRPO26 | RET1 | RPB10 | RPB8 | RPC82 | RPC34 | RPC31 Show fewer

Review For