Reference: Howell EA, et al. (1994) CDC44: a putative nucleotide-binding protein required for cell cycle progression that has homology to subunits of replication factor C. Mol Cell Biol 14(1):255-67

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Abstract


To investigate the means by which a cell regulates the progression of the mitotic cell cycle, we characterized cdc44, a mutation that causes Saccharomyces cerevisiae cells to arrest before mitosis. CDC44 encodes a 96-kDa basic protein with significant homology to a human protein that binds DNA (PO-GA) and to three subunits of human replication factor C (also called activator 1). The hypothesis that Cdc44p is involved in DNA metabolism is supported by the observations that (i) levels of mitotic recombination suggest elevated rates of DNA damage in cdc44 mutants and (ii) the cell cycle arrest observed in cdc44 mutants is alleviated by the DNA damage checkpoint mutations rad9, mec1, and mec2. The predicted amino acid sequence of Cdc44p contains GTPase consensus sites, and mutations in these regions cause a conditional cell cycle arrest. Taken together, these observations suggest that the essential CDC44 gene may encode the large subunit of yeast replication factor C.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, Non-P.H.S. | Research Support, U.S. Gov't, P.H.S.
Authors
Howell EA, McAlear MA, Rose D, Holm C
Primary Lit For
RAD9 | RFC1 | DNA replication factor C complex
Additional Lit For
MEC1 | RAD53 | rfc1-(cdc44-1) | rfc1-(cdc44-2) | rfc1-(cdc44-4) | rfc1-(cdc44-9)

Phenotype Annotations 4 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
RFC1cell cycle progression through the G2/M phase transition: arrested
homozygous diploidconditional
Allele: rfc1-(cdc44-1)

D513N

S288CTemperature: reduced temperature, 14 °C
Details: arrest as large-budded cells prior to nuclear division
RFC1cold sensitivity: increased
classical geneticsconditional
Allele: rfc1-(cdc44-1)

D513N

S288CTemperature: reduced temperature, 14 °C
RFC1mitotic recombination: increased rate
homozygous diploid conditional
Allele: rfc1-(cdc44-1)

D513N

S288CTemperature: permissive temperature, 30 °C
Details: 20-fold increase in the rate of mitotic recombination
RFC1spindle morphology: abnormal
classical geneticsconditional
Allele: rfc1-(cdc44-1)

D513N

S288CTemperature: reduced temperature, 14 °C
Details: arrest with short spindles and numerous cytoplasmic microtubules
Showing 1 to 4 of 4 entries