The yeast retroviruslike element Ty3 inserts at the transcription initiation sites of genes transcribed by RNA polymerase III (Pol III). An in vitro integration assay was developed with the use of Ty3 viruslike particles and a modified SUP2 tyrosine transfer RNA (tRNA(Tyr)) gene target. Integration was position-specific and required Ty3 integrase, Pol III transcription factor (TF) IIIB-, TFIIIC-, and Pol III-containing fractions showed that TFIIIB and TFIIIC, together, were sufficient for position-specific Ty3 integration, but not for transcription. This report demonstrates that in vitro integration of a retroelement can be targeted by cellular proteins.

• PMID: 7878467
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Reference Type

Journal Article | Research Support, U.S. Gov't, P.H.S.

Authors

Kirchner J, Connolly CM, Sandmeyer SB

Primary Lit For

General transcription factor TFIIIB complex | General transcription factor TFIIIC complex | DNA-directed RNA polymerase III complex

Additional Lit For

SUP2

Review For