Reference: Calvo O, et al. (1999) GCD14p, a repressor of GCN4 translation, cooperates with Gcd10p and Lhp1p in the maturation of initiator methionyl-tRNA in Saccharomyces cerevisiae. Mol Cell Biol 19(6):4167-81

Reference Help

Abstract


Gcd10p and Gcd14p were first identified genetically as repressors of GCN4 mRNA translation in Saccharomyces cerevisiae. Recent findings indicate that Gcd10p and Gcd14p reside in a nuclear complex required for the presence of 1-methyladenosine in tRNAs. Here we show that Gcd14p is an essential protein with predicted binding motifs for S-adenosylmethionine, consistent with a direct function in tRNA methylation. Two different gcd14 mutants exhibit defects in cell growth and accumulate high levels of initiator methionyl-tRNA (tRNAiMet) precursors containing 5' and 3' extensions, suggesting a defect in processing of the primary transcript. Dosage suppressors of gcd10 mutations, encoding tRNAiMet (hcIMT1 to hcIMT4; hc indicates that the gene is carried on a high-copy-number plasmid) or a homologue of human La protein implicated in tRNA 3'-end formation (hcLHP1), also suppressed gcd14 mutations. In fact, the lethality of a GCD14 deletion was suppressed by hcIMT4, indicating that the essential function of Gcd14p is required for biogenesis of tRNAiMet. A mutation in GCD10 or deletion of LHP1 exacerbated the defects in cell growth and expression of mature tRNAiMet in gcd14 mutants, consistent with functional interactions between Gcd14p, Gcd10p, and Lhp1p in vivo. Surprisingly, the amounts of NME1 and RPR1, the RNA components of RNases P and MRP, were substantially lower in gcd14 lhp1::LEU2 double mutants than in the corresponding single mutants, whereas 5S rRNA was present at wild-type levels. Our findings suggest that Gcd14p and Lhp1p cooperate in the maturation of a subset of RNA polymerase III transcripts.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Calvo O, Cuesta R, Anderson J, Gutiérrez N, García-Barrio MT, Hinnebusch AG, Tamame M
Primary Lit For
LHP1 | GCD14 | GCD10 | tRNA (adenine(58)-N(1))-methyltransferase complex | DNA-directed RNA polymerase III complex
Additional Lit For
RPR1 | GCN4 | NME1

Gene Ontology Annotations 5 entries for 1 gene


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
tRNA (adenine(58)-N(1))-methyltransferase complexenablestRNA (adenine(58)-N1)-methyltransferase activityIDAComplexPortal2020-10-20
tRNA (adenine(58)-N(1))-methyltransferase complexenablestRNA (adenine-N1)-methyltransferase activityIDAComplexPortal2020-10-20
tRNA (adenine(58)-N(1))-methyltransferase complexlocated innucleusIDAComplexPortal2020-10-20
tRNA (adenine(58)-N(1))-methyltransferase complexenablestRNA bindingBSRComplexPortal2020-10-20
tRNA (adenine(58)-N(1))-methyltransferase complexinvolved intRNA methylationIDAComplexPortal2020-10-20
Showing 1 to 5 of 5 entries

Interaction Annotations


Genetic Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 1 entry for 2 genes

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

InteractorInteractorAssayAnnotationActionModification
GCD14GCD10Affinity Capture-Westernmanually curatedHit-BaitNo Modification
Showing 1 to 1 of 1 entries