Reference: Rundlett SE, et al. (1996) HDA1 and RPD3 are members of distinct yeast histone deacetylase complexes that regulate silencing and transcription. Proc Natl Acad Sci U S A 93(25):14503-8

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Abstract


Increased histone acetylation has been correlated with increased transcription, and regions of heterochromatin are generally hypoacetylated. In investigating the cause-and-effect relationship between histone acetylation and gene activity, we have characterized two yeast histone deacetylase complexes. Histone deacetylase-A (HDA) is an approximately 350-kDa complex that is highly sensitive to the deacetylase inhibitor trichostatin A. Histone deacetylase-B (HDB) is an approximately 600-kDa complex that is much less sensitive to trichostatin A. The HDA1 protein (a subunit of the HDA activity) shares sequence similarity to RPD3, a factor required for optimal transcription of certain yeast genes. RPD3 is associated with the HDB activity. HDA1 also shares similarity to three new open reading frames in yeast, designated HOS1, HOS2, and HOS3. We find that both hda1 and rpd3 deletions increase acetylation levels in vivo at all sites examined in both core histones H3 and H4, with rpd3 deletions having a greater impact on histone H4 lysine positions 5 and 12. Surprisingly, both hda1 and rpd3 deletions increase repression at telomeric loci, which resemble heterochromatin with rpd3 having a greater effect. In addition, rpd3 deletions retard full induction of the PHO5 promoter fused to the reporter lacZ. These data demonstrate that histone acetylation state has a role in regulating both heterochromatic silencing and regulated gene expression.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Rundlett SE, Carmen AA, Kobayashi R, Bavykin S, Turner BM, Grunstein M
Primary Lit For
HOS2 | HDA1 | RPD3 | HOS1 | HOS3 | HDA1 histone deacetylase complex
Additional Lit For
PHO5 | SNT2C histone deacetylase complex

Gene Ontology Annotations 3 entries for 2 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
HDA1part ofHDA1 complexIDASGD2013-08-07
RPD3part ofhistone deacetylase complexIDASGD2013-08-07
RPD3enableshistone deacetylase activityIMPSGD2024-02-26
Showing 1 to 3 of 3 entries

Phenotype Annotations 3 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
HDA1protein/peptide modification: increased
Reporter: histone H3 and H4
classical geneticsnull
Allele: hda1-Δ
OtherDetails: histones H3 and H4 are hyperacetylated
HDA1silencing: increased
classical geneticsnull
Allele: hda1-Δ
OtherDetails: telomere position effect (TPE) increased
HDA1viable
classical geneticsnull
Allele: hda1-Δ
Other
Showing 1 to 3 of 3 entries