The CKS1 gene of Saccharomyces cerevisiae encodes a small essential protein shown to interact genetically and physically with the Cdc28 protein kinase. To investigate the specific functions of the CKS1 gene product, conditional temperature-sensitive mutant alleles were generated. The mutations were found to impair the ability of cells to undergo both the G1/S-phase and G2/M-phase transitions of the cell cycle, as well as the ability to bud. Mutants were not defective, however, in their ability to activate Cdc28 kinase as assayed in vitro on the substrate histone H1. It is likely, therefore, that Cks1 mediates a more specialized function of the Cdc28 kinase such as its ability to form specific multimeric complexes or to localize properly in cellular compartments.
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| CKS1 | cell cycle progression in G1 phase: arrested | classical genetics | conditional Allele: cks1-38 F8L, D36G, I79V, V145A | Other | Temperature: elevated temperature, 38.5 °C Treatment: alpha-factor block release Details: synchronized cells arrest at 38.5 deg C in G1 phase as large unbudded cells with unreplicated DNA | |
| CKS1 | cellular morphology: abnormal | classical genetics | conditional Allele: cks1-38 F8L, D36G, I79V, V145A | Other | Temperature: elevated temperature, 37 °C Treatment: alpha-factor block release Details: cells terminally arrest in G2 as extremely large unbudded cells with at least one extended process and 2N DNA content at the semi-restrictive temperature | |
| CKS1 | heat sensitivity: increased | classical genetics | conditional Allele: cks1-38 F8L, D36G, I79V, V145A | Other | Temperature: elevated temperature, 37 °C | |
| CKS1 | mitotic cell cycle: arrested | classical genetics | conditional Allele: cks1-38 F8L, D36G, I79V, V145A | Other | Temperature: elevated temperature, 38.5 °C Details: cells arrest at both the G1/S-phase and G2/M-phase transitions at the restrictive temperature; accumulation of cells with 1N and 2N complements of DNA | |
| CKS1 | mitotic cell cycle: arrested | classical genetics | conditional Allele: cks1-38 F8L, D36G, I79V, V145A | Other | Temperature: elevated temperature, 37 °C Details: initially cells accumulate at both the G1/S-phase and G2/M-phase transitions at semi-restrictive temperature; prolonged incubation results in accumulation of cells with 2N DNA content indicative of a G2/M-phase block |