A novel Saccharomyces cerevisiae mutant, unable to grow in the presence of 12.5 mM EGTA, was isolated by replica plating. The phenotype of the mutant is caused by a single amino acid change (Gly149 to Arg) in the essential yeast gene CDC1. The mutant could be suppressed by overexpression of the SMF1 gene, which was isolated as an extragenic high-copy suppressor. The SMF1 gene codes for a highly hydrophobic protein and its deletion renders the yeast cells sensitive to low manganese concentration. In accordance with this observation, the smf1 null mutant exhibits reduced Mn2+ uptake at micromolar concentrations. Using a specific antibody, we demonstrated that Smf1p is located in the yeast plasma membrane. These results suggest that Smf1p is involved in high-affinity Mn2+ uptake. This assumption was also tested by overexpressing the SMF1 gene in the temperature-sensitive mutant of the mitochondrial processing peptidase (MAS1). SMF1 overexpression as well as addition of 1 mM Mn2+ to the growth medium complemented this mutation. This also suggests that in vivo Mas1p is a manganese-dependent peptidase. The yeast Smf1p resembles a protein from Drosophila and mammalian macrophages. The latter was implicated in conferring resistance to mycobacteria. A connection between Mn2+ transport and resistance or sensitivity to mycobacteria is discussed.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| SMF1 | located in | plasma membrane | IDA | SGD | 2013-08-07 | |
| SMF1 | involved in | intracellular manganese ion homeostasis | IMP | SGD | 2013-08-07 | |
| SMF1 | involved in | manganese ion transport | IMP | SGD | 2013-08-07 |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| SMF1 | chemical compound accumulation: decreased | classical genetics | null Allele: smf1-Δ | W303 | manganese(2+) | |
| SMF1 | chemical compound accumulation: increased | classical genetics | overexpression | W303 | manganese(2+) | |
| CDC1 | inviable | classical genetics | null Allele: cdc1-Δ | W303 | ||
| SMF1 | metal resistance: decreased | classical genetics | null Allele: smf1-Δ | W303 | 2 mM nickel dichloride | |
| SMF1 | resistance to chemicals: decreased | classical genetics | null Allele: smf1-Δ | W303 | 12.5 uM ethylene glycol bis(2-aminoethyl)tetraacetic acid | |
| SMF1 | viable | classical genetics | null Allele: smf1-Δ | W303 |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.