Cdc13 and Est1 are single-strand telomeric DNA binding proteins that contribute to telomere replication in the yeast Saccharomyces cerevisiae. Here it is shown that fusion of Cdc13 to the telomerase-associated Est1 protein results in greatly elongated telomeres. Fusion proteins consisting of mutant versions of Cdc13 or Est1 confer similar telomere elongation, indicating that close physical proximity can bypass telomerase-defective mutations in either protein. Fusing Cdc13 directly to the catalytic core of telomerase allows stable telomere maintenance in the absence of Est1, consistent with a role for Est1 in mediating telomerase access. Telomere length homeostasis therefore is maintained in part by restricting access of telomerase to chromosome termini, but this limiting situation can be overcome by directly tethering telomerase to the telomere.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| Telomerase holoenzyme complex | enables | telomerase RNA reverse transcriptase activity | BSR | ComplexPortal | 2016-03-18 | |
| Telomerase holoenzyme complex | enables | template for synthesis of G-rich strand of telomere DNA activity | BSR | ComplexPortal | 2016-03-18 | |
| Telomerase holoenzyme complex | involved in | telomere maintenance via telomerase | BSR | ComplexPortal | 2016-03-18 | |
| Telomerase holoenzyme complex | enables | telomerase activity | BSR | ComplexPortal | 2016-03-18 |