Reference: Kamura T, et al. (1999)
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Abstract
The von Hippel-Lindau (VHL) tumor suppressor gene is mutated in most human kidney cancers. The VHL protein is part of a complex that includes Elongin B, Elongin C, and Cullin-2, proteins associated with transcriptional elongation and ubiquitination. Here it is shown that the endogenous VHL complex in rat liver also includes Rbx1, an evolutionarily conserved protein that contains a RING-H2 fingerlike motif and that interacts with Cullins. The yeast homolog of Rbx1 is a subunit and potent activator of the Cdc53-containing SCFCdc4 ubiquitin ligase required for ubiquitination of the cyclin-dependent kinase inhibitor Sic1 and for the G1 to S cell cycle transition. These findings provide a further link between VHL and the cellular ubiquitination machinery.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, P.H.S.
- Authors
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Kamura T,
Koepp DM,
Conrad MN,
Skowyra D,
Moreland RJ,
Iliopoulos O,
Lane WS,
Kaelin WG,
Elledge SJ,
Conaway RC,
... Show all
Harper JW,
Conaway JW
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- HRT1
- SCF-Cdc4 ubiquitin ligase complex | SCF-Ydr131c ubiquitin ligase complex
Gene Ontology Annotations
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| Evidence ID |
Analyze ID |
Gene/Complex |
Systematic Name/Complex Accession |
Qualifier |
Gene Ontology Term ID |
Gene Ontology Term |
Aspect |
Annotation Extension |
Evidence |
Method |
Source |
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Reference |
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| Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
Strain Background |
Chemical |
Details |
Reference |
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| Evidence ID |
Analyze ID |
Gene |
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Disease Ontology Term |
Disease Ontology Term ID |
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Reference |
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| Evidence ID |
Analyze ID |
Regulator |
Regulator Systematic Name |
Target |
Target Systematic Name |
Direction |
Regulation of |
Happens During |
Regulator Type |
Direction |
Regulation Of |
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Post-translational Modifications
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Genetic Interactions
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| Evidence ID |
Analyze ID |
|
Interactor |
Interactor Systematic Name |
Interactor |
Interactor Systematic Name |
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Assay |
Annotation |
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Note |
Physical Interactions
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
|---|
| CDC4 | CDC53 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| CDC4 | HRT1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| CDC4 | HRT1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| CDC4 | SKP1 | Affinity Capture-Western | manually curated | Bait-Hit | No Modification |
| CDC53 | HRT1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
| SKP1 | HRT1 | Affinity Capture-Western | manually curated | Hit-Bait | No Modification |
Functional Complementation Annotations
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| Gene | Species | Gene ID | Strain background | Direction | Details | Source |
|---|
| HRT1 | Homo sapiens | HGNC:9928 | S288C | other complements yeast | | SGD |