We have cloned a gene (BCY1) from the yeast Saccharomyces cerevisiae that encodes a regulatory subunit of the cyclic AMP-dependent protein kinase. The encoded protein has a structural organization similar to that of the RI and RII regulatory subunits of the mammalian cyclic AMP-dependent protein kinase. Strains of S. cerevisiae with disrupted BCY1 genes do not display a cyclic AMP-dependent protein kinase in vitro, fail to grow on many carbon sources, and are exquisitely sensitive to heat shock and starvation.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| BCY1 | innate thermotolerance: decreased | classical genetics | null Allele: bcy1-Δ | Other | Treatment: heat shock, 55 °C | |
| BCY1 | spore germination: decreased | classical genetics | null Allele: bcy1-Δ | Other | ||
| BCY1 | sporulation: absent | homozygous diploid | null Allele: bcy1-Δ | Other | ||
| BCY1 | starvation resistance: decreased | classical genetics | null Allele: bcy1-Δ | Other | Media: nitrogen starvation Details: decreased survival after nitrogen starvation | |
| BCY1 | utilization of carbon source: absent | classical genetics | null Allele: bcy1-Δ | Other | 2% potassium acetate | Details: similar results with raffinose, galactose, glycerol, and pyruvate |