Reference: Toda T, et al. (1987) Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Mol Cell Biol 7(4):1371-7

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Abstract


We have cloned a gene (BCY1) from the yeast Saccharomyces cerevisiae that encodes a regulatory subunit of the cyclic AMP-dependent protein kinase. The encoded protein has a structural organization similar to that of the RI and RII regulatory subunits of the mammalian cyclic AMP-dependent protein kinase. Strains of S. cerevisiae with disrupted BCY1 genes do not display a cyclic AMP-dependent protein kinase in vitro, fail to grow on many carbon sources, and are exquisitely sensitive to heat shock and starvation.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Toda T, Cameron S, Sass P, Zoller M, Scott JD, McMullen B, Hurwitz M, Krebs EG, Wigler M
Primary Lit For
TPK3 | BCY1 | TPK1 | TPK2 | cAMP-dependent protein kinase complex variant 1 | cAMP-dependent protein kinase complex variant 2 | cAMP-dependent protein kinase complex variant 3 | cAMP-dependent protein kinase complex variant 4 | cAMP-dependent protein kinase complex variant 5 | cAMP-dependent protein kinase complex variant 6

Gene Ontology Annotations


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Gene/Complex Qualifier Gene Ontology Term Aspect Annotation Extension Evidence Method Source Assigned On Reference

Phenotype Annotations 5 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
BCY1innate thermotolerance: decreased
classical geneticsnull
Allele: bcy1-Δ
OtherTreatment: heat shock, 55 °C
BCY1spore germination: decreased
classical geneticsnull
Allele: bcy1-Δ
Other
BCY1sporulation: absent
homozygous diploidnull
Allele: bcy1-Δ
Other
BCY1starvation resistance: decreased
classical geneticsnull
Allele: bcy1-Δ
OtherMedia: nitrogen starvation
Details: decreased survival after nitrogen starvation
BCY1utilization of carbon source: absent
classical geneticsnull
Allele: bcy1-Δ
Other2% potassium acetateDetails: similar results with raffinose, galactose, glycerol, and pyruvate
Showing 1 to 5 of 5 entries