Reference: Bard M, et al. (1996) Cloning and characterization of ERG25, the Saccharomyces cerevisiae gene encoding C-4 sterol methyl oxidase. Proc Natl Acad Sci U S A 93(1):186-90

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Abstract


We have cloned the Saccharomyces cerevisiae C-4 sterol methyl oxidase ERG25 gene. The sterol methyl oxidase performs the first of three enzymic steps required to remove the two C-4 methyl groups leading to cholesterol (animal), ergosterol (fungal), and stigmasterol (plant) biosynthesis. An ergosterol auxotroph, erg25, which fails to demethylate and concomitantly accumulates 4,4-dimethylzy-mosterol, was isolated after mutagenesis. A complementing clone consisting of a 1.35-kb Dra I fragment encoded a 309-amino acid polypeptide (calculated molecular mass, 36.48 kDa). The amino acid sequence shows a C-terminal endoplasmic reticulum retrieval signal KKXX and three histidine-rich clusters found in eukaryotic membrane desaturases and in a bacterial alkane hydroxylase and xylene monooxygenase. The sterol profile of an ERG25 disruptant was consistent with the erg25 allele obtained by mutagenesis.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, Non-P.H.S. | Research Support, U.S. Gov't, P.H.S.
Authors
Bard M, Bruner DA, Pierson CA, Lees ND, Biermann B, Frye L, Koegel C, Barbuch R
Primary Lit For
ERG25 | ERG29

Gene Ontology Annotations 3 entries for 2 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
ERG25enablesC-4 methylsterol oxidase activityhas input 14-demethyllanosterol, has output 4beta-methylzymosterol-4alpha-carboxylate, part of ergosterol biosynthetic processIMPUniProt2022-07-07
ERG25involved inergosterol biosynthetic processIMPUniProt2022-07-07
ERG29acts upstream of or withinergosterol biosynthetic processIMPUniProt2022-07-07
Showing 1 to 3 of 3 entries

Phenotype Annotations 2 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
ERG25auxotrophy
classical geneticsnull
Allele: erg25-Δ
Other ergosterol
ERG25chemical compound accumulation: increased
classical geneticsnull
Allele: erg25-Δ
Other 14-demethyllanosterolDetails: accumulation of 4,4-dimethylzymosterol and other C-4,4-dimethylsterols
Showing 1 to 2 of 2 entries