Reference: Kerscher O, et al. (2000) Tim18p is a new component of the Tim54p-Tim22p translocon in the mitochondrial inner membrane. Mol Biol Cell 11(1):103-16

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Abstract


The mitochondrial inner membrane contains two separate translocons: one required for the translocation of matrix-targeted proteins (the Tim23p-Tim17p complex) and one for the insertion of polytopic proteins into the mitochondrial inner membrane (the Tim54p-Tim22p complex). To identify new members of the Tim54p-Tim22p complex, we screened for high-copy suppressors of the temperature-sensitive tim54-1 mutant. We identified a new gene, TIM18, that encodes an integral protein of the inner membrane. The following genetic and biochemical observations suggest that the Tim18 protein is part of the Tim54p-Tim22p complex in the inner membrane: multiple copies of TIM18 suppress the tim54-1 growth defect; the tim18::HIS3 disruption is synthetically lethal with tim54-1; Tim54p and Tim22p can be coimmune precipitated with the Tim18 protein; and Tim18p, along with Tim54p and Tim22p, is detected in an approximately 300-kDa complex after blue native electrophoresis. We propose that Tim18p is a new component of the Tim54p-Tim22p machinery that facilitates insertion of polytopic proteins into the mitochondrial inner membrane.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Kerscher O, Sepuri NB, Jensen RE
Primary Lit For
TIM22 | TIM54 | SHH4 | TIM18 | TIM22 mitochondrial inner membrane twin-pore carrier translocase complex

Gene Ontology Annotations 6 entries for 4 genes


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
TIM22part ofTIM22 mitochondrial import inner membrane insertion complexIDASGD2013-08-07
TIM54part ofTIM22 mitochondrial import inner membrane insertion complexIDASGD2013-08-07
TIM54involved inprotein insertion into mitochondrial inner membraneIGI with TIM18SGD2013-08-07
SHH4located inmitochondrial inner membraneIDASGD2013-08-07
TIM18part ofTIM22 mitochondrial import inner membrane insertion complexIDASGD2013-08-07
TIM18involved inprotein insertion into mitochondrial inner membraneIGI with TIM54SGD2013-08-07
Showing 1 to 6 of 6 entries

Phenotype Annotations 8 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
TIM18cold sensitivity: increased
classical geneticsnull
Allele: tim18-Δ
OtherMedia: glucose medium
Temperature: reduced temperature, 24 °C
TIM18inviable
classical geneticsnull
Allele: tim18-Δ
OtherMedia: glucose medium
TIM18mitochondrial transport: decreased
Reporter: Cox4p
classical geneticsnull
Allele: tim18-Δ
Other
TIM18mitochondrial transport: decreased
Reporter: phosphate carrier (PiC)
classical geneticsnull
Allele: tim18-Δ
Other
TIM18mitochondrial transport: decreased
Reporter: Tim22p
classical geneticsnull
Allele: tim18-Δ
Other
TIM18petite-negative
classical geneticsnull
Allele: tim18-Δ
Other
TIM18respiratory growth: decreased rate
classical geneticsnull
Allele: tim18-Δ
Other
TIM18vegetative growth: decreased rate
classical geneticsnull
Allele: tim18-Δ
Other
Showing 1 to 8 of 8 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 2 entries for 3 genes

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InteractorInteractorAssayAnnotationActionModification
TIM22TIM18Affinity Capture-Westernmanually curatedHit-BaitNo Modification
TIM54TIM18Affinity Capture-Westernmanually curatedHit-BaitNo Modification
Showing 1 to 2 of 2 entries