The mitochondrial inner membrane contains two separate translocons: one required for the translocation of matrix-targeted proteins (the Tim23p-Tim17p complex) and one for the insertion of polytopic proteins into the mitochondrial inner membrane (the Tim54p-Tim22p complex). To identify new members of the Tim54p-Tim22p complex, we screened for high-copy suppressors of the temperature-sensitive tim54-1 mutant. We identified a new gene, TIM18, that encodes an integral protein of the inner membrane. The following genetic and biochemical observations suggest that the Tim18 protein is part of the Tim54p-Tim22p complex in the inner membrane: multiple copies of TIM18 suppress the tim54-1 growth defect; the tim18::HIS3 disruption is synthetically lethal with tim54-1; Tim54p and Tim22p can be coimmune precipitated with the Tim18 protein; and Tim18p, along with Tim54p and Tim22p, is detected in an approximately 300-kDa complex after blue native electrophoresis. We propose that Tim18p is a new component of the Tim54p-Tim22p machinery that facilitates insertion of polytopic proteins into the mitochondrial inner membrane.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| TIM22 | part of | TIM22 mitochondrial import inner membrane insertion complex | IDA | SGD | 2013-08-07 | |
| TIM54 | part of | TIM22 mitochondrial import inner membrane insertion complex | IDA | SGD | 2013-08-07 | |
| TIM54 | involved in | protein insertion into mitochondrial inner membrane | IGI with TIM18 | SGD | 2013-08-07 | |
| SHH4 | located in | mitochondrial inner membrane | IDA | SGD | 2013-08-07 | |
| TIM18 | part of | TIM22 mitochondrial import inner membrane insertion complex | IDA | SGD | 2013-08-07 | |
| TIM18 | involved in | protein insertion into mitochondrial inner membrane | IGI with TIM54 | SGD | 2013-08-07 |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| TIM18 | cold sensitivity: increased | classical genetics | null Allele: tim18-Δ | Other | Media: glucose medium Temperature: reduced temperature, 24 °C | |
| TIM18 | inviable | classical genetics | null Allele: tim18-Δ | Other | Media: glucose medium | |
| TIM18 | mitochondrial transport: decreased Reporter: Cox4p | classical genetics | null Allele: tim18-Δ | Other | ||
| TIM18 | mitochondrial transport: decreased Reporter: phosphate carrier (PiC) | classical genetics | null Allele: tim18-Δ | Other | ||
| TIM18 | mitochondrial transport: decreased Reporter: Tim22p | classical genetics | null Allele: tim18-Δ | Other | ||
| TIM18 | petite-negative | classical genetics | null Allele: tim18-Δ | Other | ||
| TIM18 | respiratory growth: decreased rate | classical genetics | null Allele: tim18-Δ | Other | ||
| TIM18 | vegetative growth: decreased rate | classical genetics | null Allele: tim18-Δ | Other |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.