Reference: Svejstrup JQ, et al. (1994)
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Abstract
An RNA polymerase transcription factor IIH holoenzyme (holoTFIIH) has been resolved to near homogeneity from Saccharomyces cerevisiae. HoloTFIIH comprises the five-subunit core transcription factor described previously (Feaver, W. J., Svejstrup, J. Q., Bardwell, A. J., Bardwell, L., Buratowski, S., Gulyas, K. D., Donahue, T. F., Friedberg, E. C. and Kornberg, R. D. (1993) Cell 75, 1379-1387) and in addition, SSL2 and three further, as yet unidentified, polypeptides. HoloTFIIH possesses C-terminal repeat domain kinase activity and, together with other pure yeast transcription proteins, enables RNA polymerase II transcription in a fully defined system. By contrast, core TFIIH is inert in both C-terminal repeat domain kinase and reconstituted transcription assays.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, P.H.S.
- Authors
-
Svejstrup JQ,
Feaver WJ,
LaPointe J,
Kornberg RD
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- SSL2 | General transcription factor TFIIH complex | DNA-directed RNA polymerase II complex
- TFB1 | KIN28 | RAD3 | SSL1 | TFB4 | TFB3 | CCL1 | TFB2
Gene Ontology Annotations
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| Evidence ID |
Analyze ID |
Gene |
Gene Systematic Name |
Phenotype |
Experiment Type |
Experiment Type Category |
Mutant Information |
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Details |
Reference |
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Disease Ontology Term |
Disease Ontology Term ID |
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Assigned On |
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Reference |
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
|---|
| RAD3 | SSL2 | Co-purification | manually curated | Bait-Hit | No Modification |
| RAD3 | SSL1 | Co-purification | manually curated | Bait-Hit | No Modification |
| RAD3 | TFB1 | Co-purification | manually curated | Bait-Hit | No Modification |
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| Complement ID |
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Details |
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Reference |