A second form (beta isoform) of rat phosphatidylinositol transfer protein cDNA was cloned by complementation of the yeast sec14 mutation from a rat brain cDNA expression library. The deduced sequence of the protein comprised 271 amino acids with a calculated molecular mass of 31,449 Da. The deduced amino acid sequence showed 77% identity to that of the rat phosphatidylinositol transfer protein, recently reported by Dickeson et al. [Dickeson, S.K., Lim, C.N., Schuyler, G.T., Dalton, T.P., Helmkamp, G.M., Jr., & Yarbrough, L.R. (1989) J. Biol. Chem. 264, 16557-16564]. Northern blot analysis revealed that the cDNA probe of the beta isoform hybridized to an approximately 3-kilobase RNA in various rat tissues. The mRNA was expressed abundantly in brain, kidney, liver, and lung, but in a lesser amount in testis.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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