In this study, we report the further characterization of the Saccharomyces cerevisiae crystal violet-resistance protein Sge1. Sge1 is a highly hydrophobic 59 kDa protein with 14 predicted membrane-spanning domains. It shares homologies with several drug-resistance proteins and sugar transporters of the major facilitator superfamily. Here, we have demonstrated that Sge1 is not only a crystal violet-resistance protein, but that it also confers resistance to ethidium bromide and methylmethane sulfonate. Disruption of SGE1 leads to increased sensitivity towards all three compounds, thus designating Sge1 as a multiple drug-resistance protein. Subcellular fractionation as well as immunolocalization on whole yeast cells demonstrated that Sge1 was tightly associated with the yeast plasma membrane. Furthermore, Sge1 was highly enriched in preparations of yeast plasma membranes. In analogy to other multidrug-resistance proteins, we suggest that Sge1 functions as a drug export permease.
Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.
| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| SGE1 | located in | plasma membrane | IDA | SGD | 2022-08-26 | |
| SGE1 | enables | xenobiotic transmembrane transporter activity | has input methyl methanesulfonate | IMP | SGD | 2019-04-18 |
| SGE1 | enables | xenobiotic transmembrane transporter activity | has input crystal violet | IMP | SGD | 2019-04-18 |
| SGE1 | enables | xenobiotic transmembrane transporter activity | has input ethidium bromide | IMP | SGD | 2019-04-18 |
| SGE1 | involved in | transmembrane transport | has input methyl methanesulfonate | IMP | SGD | 2019-04-18 |
| SGE1 | involved in | transmembrane transport | has input crystal violet | IMP | SGD | 2019-04-18 |
| SGE1 | involved in | transmembrane transport | has input ethidium bromide | IMP | SGD | 2019-04-18 |