Reference: Kamura T, et al. (1999)
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Abstract
The RING-H2 finger protein Rbx1 is a subunit of the related SCF (Skp1-Cdc53/Cul1-F-box protein) and von Hippel-Lindau (VHL) tumor suppressor (elongin BC-Cul2-VHL) E3 ubiquitin ligase complexes, where it functions as a component of Cdc53/Rbx1 and Cul2/Rbx1 modules that activate ubiquitination of target proteins by the E2 ubiquitin-conjugating enzymes Cdc34 and Ubc5. Here we demonstrate that the Cdc53/Rbx1 and Cul2/Rbx1 modules also activate conjugation of the ubiquitin-like protein Rub1 to Cdc53 and Cul2 by the dedicated E2 Rub1 conjugating enzyme Ubc12. Our findings identify Rbx1 as a common component of enzyme systems responsible for ubiquitin and Rub1 modification of target proteins.
- Reference Type
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Comparative Study |
Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, P.H.S.
- Authors
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Kamura T,
Conrad MN,
Yan Q,
Conaway RC,
Conaway JW
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- HRT1
Gene Ontology Annotations
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
|---|
| GRR1 | CLN2 | Biochemical Activity | manually curated | Bait-Hit | ubiquitinylated lysine |
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