Reference: Shoji K, et al. (1998) Cloning of a cDNA encoding an importin-alpha and down-regulation of the gene by light in rice leaves. Gene 212(2):279-86

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Abstract


The import of nuclear proteins into nuclei begins with recognition of nuclear localization signal-harboring proteins and binding to a nuclear pore targeting complex. A cDNA for an importin-alpha protein, a subunit of the complex, was isolated from rice plants. The amino acid sequence deduced from the nucleotide sequence of the cDNA exhibited a high homology to those of importin-alpha proteins from many organisms such as Arabidopsis thaliana, Saccharomyces cerevisiae, human, mouse, Xenopus laevis and Drosophila melanogaster. Down-regulation of the transcription by light was shown in the leaves of light- and dark-grown seedlings by RNA blot analysis. The down-regulation was specific to leaves, whereas no light effect was observed in root tissues or calli, in which higher levels of the transcript were detected.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Shoji K, Iwasaki T, Matsuki R, Miyao M, Yamamoto N
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