Reference: Liu Y, et al. (2000) Characterization of a Saccharomyces cerevisiae homologue of Schizosaccharomyces pombe Chk1 involved in DNA-damage-induced M-phase arrest. Mol Gen Genet 262(6):1132-46

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Abstract


Chk1 is an evolutionarily conserved protein kinase that plays an essential role in mediating G2 arrest in response to DNA damage in Schizosaccharomyces pombe and human cells. It functions by maintaining the inhibition (by phosphorylation of a specific tyrosine residue) of the cyclin-dependent kinase Cdc2 that initiates the G2/M transition. Here, we characterize a structural homologue of Chk1 in the budding yeast Saccharomyces cerevisiae. In this organism, G2/M arrest following DNA damage is considered to be independent of tyrosine phosphorylation of the Cdc2 homologue Cdc28. Nevertheless, a partial defect in G2/M-phase arrest following treatment with ionizing radiation, but not UV radiation, is associated with deletion of CHK1. The fact that such an effect remains detectable in cells synchronized with the microtubule inhibitor nocodazole prior to gamma irradiation implies the existence of a CHK1-dependent checkpoint in M phase. We conclude from epistasis analysis that Chk1 participates in the Pds1-dependent subpathway of M-phase arrest. In spite of the partial checkpoint defect of the chk1 mutant, the survival of colony-forming cells is not notably decreased following UV and gamma irradiation. In two-hybrid screens, we identified a heme-binding stress protein (encoded by the yeast ORF YNL234W), a protein involved in genomic silencing (Sas3) and Chk1 itself as interacting partners of Chk1.

Reference Type
Comparative Study | Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Liu Y, Vidanes G, Lin YC, Mori S, Siede W
Primary Lit For
CHK1
Additional Lit For
SAS3 | YNL234W | CDC28 | TCO89

Gene Ontology Annotations 1 entry for 1 gene


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
CHK1involved inDNA damage checkpoint signalingIMPSGD2013-08-07
Showing 1 to 1 of 1 entries

Phenotype Annotations 2 entries for 1 gene


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GenePhenotypeExperiment TypeMutant InformationStrain BackgroundChemicalDetails
CHK1cell cycle progression through the G2/M phase transition: abnormal
classical geneticsnull
Allele: chk1-Δ
OtherRadiation: treatment with ionizing radiation
Details: abbreviated cell cycle arrest in response to DNA damage
CHK1cell cycle progression through the G2/M phase transition: normal
classical geneticsnull
Allele: chk1-Δ
OtherRadiation: UV irradiation
Details: normal cell cycle arrest in response to DNA damage
Showing 1 to 2 of 2 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 3 entries for 3 genes

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InteractorInteractorAssayAnnotationActionModification
CHK1CHK1Two-hybridmanually curatedHit-BaitNo Modification
CHK1YNL234WTwo-hybridmanually curatedBait-HitNo Modification
SAS3CHK1Two-hybridmanually curatedHit-BaitNo Modification
Showing 1 to 3 of 3 entries