Reference: Michaelis C, et al. (1997)
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Abstract
Cohesion between sister chromatids opposes the splitting force exerted by microtubules, and loss of this cohesion is responsible for the subsequent separation of sister chromatids during anaphase. We describe three chromosmal proteins that prevent premature separation of sister chromatids in yeast. Two, Smc1p and Smc3p, are members of the SMC family, which are putative ATPases with coiled-coil domains. A third protein, which we call Scc1p, binds to chromosomes during S phase, dissociates from them at the metaphase-to-anaphase transition, and is degraded by the anaphase promoting complex. Association of Scc1p with chromatin depends on Smc1p. Proteins homologous to Scc1p exist in a variety of eukaryotic organisms including humans. A common cohesion apparatus might be used by all eukaryotic cells during both mitosis and meiosis.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't
- Authors
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Michaelis C,
Ciosk R,
Nasmyth K
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- MCD1 | SCC2 | SMC1 | SMC3
- smc3-42 | mcd1-73 | scc2-4
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|
| MCD1 | chromosome segregation: abnormal
| classical genetics | conditional
Allele: mcd1-73 S525N; winged helix domain (WHD) recognition helix mutated | W303 | | Temperature: elevated temperature, 37 °C
Treatment: nocodazole treated
Details: sister chromatids separate after damage to the mitotic spindle (69% vs. 17% in wt)
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| MCD1 | chromosome segregation: abnormal
| classical genetics | conditional
Allele: mcd1-73 S525N; winged helix domain (WHD) recognition helix mutated | W303 | | Temperature: elevated temperature, 37 °C
Details: sister chromatids separate prematurely (30 min. vs 45 min. in wt) in synchronized cultures
|
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