In the yeast Saccharomyces cerevisiae, the products of two genes (PSD1 and PSD2) are able to catalyze the decarboxylation of phosphatidylserine (PS) to produce phosphatidylethanolamine (PE) (C. J. Clancey, S. Chang, and W. Dowhan, J. Biol. Chem. 268:24580-24590, 1993; P. J. Trotter, J. Pedretti, and D. R. Voelker, J. Biol. Chem. 268:21416-21424, 1993; P.J. Trotter, and D. R. Voelker, J. Biol. Chem. 270:6062-6070, 1995). I report that the major mitochondrial PS decarboxylase gene (PSD1) is transcriptionally regulated by inositol in a manner similar to that reported for other coregulated phospholipid biosynthetic genes. The second PS decarboxylase gene (PSD2) is not regulated on a transcriptional level by inositol and/or ethanolamine. In yeast, phosphatidylcholine (PC) biosynthesis is required for the repression of the phospholipid biosynthetic genes, including the INO1 gene, in response to inositol. I show that the presence of a functional major mitochondrial PS decarboxylase encoded by the PSD1 gene is necessary for proper regulation of INO1 in response to inositol in the absence of ethanolamine. Disruption of the second PS decarboxylase gene (PSD2) does not affect the INO1 regulation. Analysis of phospholipid content of PS decarboxylase mutants suggests that the proportion of PC on total cellular phospholipids is not correlated to the cell's ability to repress INO1 in response to inositol. Rather, yeast cells are apparently able to monitor the flux through the phospholipid biosynthetic pathway and modify the transcription of phospholipid biosynthetic genes accordingly.

• PMID: 9294443
• DOI full text
• PMC full text
• PubMed
• PubTator

Reference Type

Journal Article | Research Support, Non-U.S. Gov't

Authors

Griac P

Primary Lit For

Additional Lit For

Review For