Reference: Kennedy BK, et al. (1997)
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Abstract
A prior genetic study indicated that activity of Sir silencing proteins at a hypothetical AGE locus is essential for long life span. In this model, the SIR4-42 mutation would direct the Sir protein complex to the AGE locus, giving rise to a long life span. We show by indirect immunofluorescence that Sir3p and Sir4p are redirected to the nucleolus in the SIR4-42 mutant. Furthermore, this relocalization is dependent on both UTH4 a novel yeast gene that extends life span, and its homologue YGL023. Strikingly, the Sir complex is relocalized from telomeres to the nucleolus in old wild-type cells. We propose that the rDNA is the AGE locus and that nucleolar function is compromised in old yeast cells in a way that may be mitigated by targeting of Sir proteins to the nucleolus.
- Reference Type
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Journal Article |
Research Support, Non-U.S. Gov't |
Research Support, U.S. Gov't, P.H.S.
- Authors
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Kennedy BK,
Gotta M,
Sinclair DA,
Mills K,
McNabb DS,
Murthy M,
Pak SM,
Laroche T,
Gasser SM,
Guarente L
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| Interactor | Interactor | Assay | Annotation | Action | Modification |
Proteins relocalize in SIR4-42 | SIR4 | SIR3 | Co-localization | manually curated | Hit-Bait | No Modification |
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