Reference: Shpakov AO (1996) [A theoretical analysis of the primary structure of the yeast SEC59 gene product. Topology in the membrane, identification of the repetitive sequences and internal symmetry]. Zh Evol Biokhim Fiziol 32(5):545-55

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Abstract


In this investigation the results of the theoretical analysis of primary structure of yeast gene SEC59 product, which possesses the dolichol kinase activity, were represented. The amino acid sequences (AAS), potentially penetrating the membrane, were identified by using of hydropathic and hydrophobic analyses. The some of these sequences were homologous. The longer symmetrical AAS with centres of symmetry in dolichol-binding region and related sequences were identified by using of comparative analysis of amino acid codon roots. The graphic method of analysis of amino acid codon roots sequences was used for identification of repeating segments in primary structure of SEC59 molecule. These segments contain less than 18 amino acid residues and construct the main part of polypeptide chain (for exclusion of N-terminal region of SEC59).

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Comparative Study | Journal Article
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Shpakov AO
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