Cells of Saccharomyces cerevisiae were permeabilized by ether for the isolation of coenzyme NADH. A 4-fold increase in the ether fraction to aqueous fraction resulted in the recovery of 80% of total NADH present in the cell. NADH was separated and purified by affinity ultrafiltration using yeast alcohol dehydrogenase as an affinity ligand. The binding characteristics of the enzyme and coenzyme were established at different pH and ionic strengths using gel filtration. The number of moles of NADH bound per mole of alcohol dehydrogenase (r) was found to be 5.7 at pH 8 and ionic strength (I) 0.1 M. The binary complex of NADH and alcohol dehydrogenase was cleaved by lowering the pH to 6.0. The crude cell permeate on purification by ultrafiltration with 2-fold dilution, gave NADH with an absorbance ratio (A260/A340) of 2.3 and overall yield of 68%. Alcohol dehydrogenase was recovered as retentate with 93% recovery and 15% loss in activity.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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