Reference: Papatsenko DA, et al. (1996) Mapping of DNA-binding proteins along the yeast genome by UV-induced DNA-protein crosslinking. FEBS Lett 381(1-2):103-5

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Abstract


UV-induced crosslinking of DNA-binding proteins to DNA in intact nuclei of Saccharomyces cerevisiae and subsequent 'protein image' hybridization were applied to map non-histone proteins along single-copy genes of yeast. We detected two polypeptides that most probably correspond to core subunits of yeast RNA-polymerase II in the coding region of transketolase gene (TKL2). Several non-histone proteins were also detected which bind to the upstream region of TKL2 gene, and to the intergenic spacer between calmodulin (CMD1) and beta-mannosyl transferase (ALG1) genes.

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Journal Article | Research Support, Non-U.S. Gov't
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Papatsenko DA, Priporova IV, Belikov SV, Karpov VL
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