Here we report the identification of BET3, a new member of a group of interacting genes whose products have been implicated in the targeting and fusion of endoplasmic reticulum (ER) to Golgi transport vesicles with their acceptor compartment. A temperature-sensitive mutant in bet3-1 was isolated in a synthetic lethal screen designed to identify new genes whose products may interact with BET1, a type II integral membrane protein that is required for ER to Golgi transport. At 37 degrees C, bet3-1 fails to transport invertase, alpha-factor, and carboxypeptidase Y from the ER to the Golgi complex. As a consequence, this mutant accumulates dilated ER and small vesicles. The SNARE complex, a docking/fusion complex, fails to form in this mutant. Furthermore, BET3 encodes an essential 22-kDa hydrophilic protein that is conserved in evolution, which is not a component of this complex. These findings support the hypothesis that Bet3p may act before the assembly of the SNARE complex.
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| Gene/Complex | Qualifier | Gene Ontology Term | Annotation Extension | Evidence | Source | Assigned On |
|---|---|---|---|---|---|---|
| BET3 | involved in | endoplasmic reticulum to Golgi vesicle-mediated transport | IMP | SGD | 2013-08-07 | |
| BET3 | contributes to | guanyl-nucleotide exchange factor activity | IGI with YPT1 | SGD | 2020-10-25 | |
| BET3 | involved in | endoplasmic reticulum to Golgi vesicle-mediated transport | IGI with YPT1 | SGD | 2013-08-07 | |
| BET3 | involved in | endoplasmic reticulum to Golgi vesicle-mediated transport | IGI with SEC21 | SGD | 2013-08-07 | |
| BET3 | involved in | endoplasmic reticulum to Golgi vesicle-mediated transport | IGI with SEC17 | SGD | 2013-08-07 | |
| BET3 | involved in | endoplasmic reticulum to Golgi vesicle-mediated transport | IGI with SEC18 | SGD | 2013-08-07 | |
| BET3 | involved in | endoplasmic reticulum to Golgi vesicle-mediated transport | IGI with BOS1 | SGD | 2013-08-07 | |
| BET3 | involved in | endoplasmic reticulum to Golgi vesicle-mediated transport | IGI with BET1 | SGD | 2013-08-07 | |
| BET3 | involved in | endoplasmic reticulum to Golgi vesicle-mediated transport | IGI with SEC22 | SGD | 2013-08-07 |
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| Gene | Phenotype | Experiment Type | Mutant Information | Strain Background | Chemical | Details |
|---|---|---|---|---|---|---|
| BET3 | endomembrane system morphology: abnormal | classical genetics | conditional Allele: bet3-1 | Other | Temperature: elevated temperature, 37 °C Details: accumulates small 60 nm secretory vesicles, as well as some large 110 nm vesicles | |
| BET3 | endoplasmic reticulum morphology: abnormal | classical genetics | conditional Allele: bet3-1 | Other | Temperature: elevated temperature, 37 °C Details: elaborated ER membrane, with dilated lumen, and often multi-lobbed nucleus | |
| BET3 | inviable | classical genetics | null Allele: bet3-Δ | Other | ||
| BET3 | protein transport: decreased Reporter: invertase | classical genetics | conditional Allele: bet3-1 | Other | Temperature: elevated temperature, 37 °C Details: similar defect observed with alpha-factor and carboxypeptidase Y (CPY) |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.