Reference: Choi KY, et al. (1994) Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell 78(3):499-512

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Abstract


Ste5 is a Zn2+ finger-like protein thought to function before three kinases, Ste11 (a MEKK), Ste7 (a MEK), and Fus3 (a MAPK), in a conserved MAP kinase cascade required for mating in S. cerevisiae. Here, we present evidence that Ste5 forms a multikinase complex that joins these kinases for efficient Fus3 activation. By two-hybrid analysis, Ste11, Ste7, and Fus3 associate with different domains of Ste5, while Kss1, another MAPK, associates with the same domain as Fus3, thus implying that Ste5 simultaneously binds a MEKK, MEK, and MAPK. Ste5 copurifies with Ste11, Fus3, and a hypophosphorylated form of Ste7, and all four proteins cosediment in a glycerol gradient as if in a large complex. Ste5 also increases the amount of Ste11 complexed to Ste7 and Fus3 and is required for Ste11 to function. These results substantiate a novel signal transduction component that physically links multiple kinases within a single cascade.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Choi KY, Satterberg B, Lyons DM, Elion EA
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Gene Ontology Annotations 2 entries for 1 gene


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Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
STE5involved inpheromone-dependent signal transduction involved in conjugation with cellular fusionIDASGD2013-08-07
STE5enablesMAP-kinase scaffold activityIDASGD2018-06-04
Showing 1 to 2 of 2 entries

Interaction Annotations


Genetic Interactions

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 6 entries for 5 genes

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InteractorInteractorAssayAnnotationActionModification
FUS3STE5Two-hybridmanually curatedHit-BaitNo Modification
FUS3STE11Co-fractionationmanually curatedHit-BaitNo Modification
STE5KSS1Two-hybridmanually curatedBait-HitNo Modification
STE5STE11Two-hybridmanually curatedBait-HitNo Modification
STE5STE11Co-fractionationmanually curatedHit-BaitNo Modification
STE7STE5Two-hybridmanually curatedHit-BaitNo Modification
Showing 1 to 6 of 6 entries