Reference: Straffon AF, et al. (1994) Rescue of yeast defective in mitochondrial ATP synthase subunit 8 by a heterologous gene from Aspergillus nidulans. Biochem Biophys Res Commun 203(3):1567-73

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Abstract


Mitochondrial ATP synthase subunit 8 of the yeast Saccharomyces cerevisiae and of the filamentous fungus Aspergillus nidulans have the same length and similar structural motifs. However, the two proteins share only 50% identical residues, with the conserved residues being concentrated in the N- and C-terminal domains. We have investigated whether it is amino acid sequence or overall structural motifs that are required for subunit 8 function. PCR was used to construct a gene encoding A. nidulans subunit 8 fused to an N-terminal cleavable mitochondrial targeting sequence. Following expression in the nucleus of a yeast strain deficient in subunit 8, the chimaeric precursor targeted the subunit 8 protein back to the mitochondrion. The A. nidulans subunit 8 was found to be able to restore growth on non-fermentable substrate at 18 degrees C and 28 degrees C, but not at 36 degrees C. Given the sequence divergence between subunit 8 of A. nidulans and that of S. cerevisiae, this finding suggests that common structural motifs are important for subunit 8 function.

Reference Type
Comparative Study | Journal Article | Research Support, Non-U.S. Gov't
Authors
Straffon AF, Nagley P, Devenish RJ
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