Reference: Preker PJ, et al. (1995) The FIP1 gene encodes a component of a yeast pre-mRNA polyadenylation factor that directly interacts with poly(A) polymerase. Cell 81(3):379-89

Reference Help

Abstract


We have identified an essential gene, called FIP1, encoding a 327 amino acid protein interacting with yeast poly(A) polymerase (PAP1) in the two-hybrid assay. Recombinant FIP1 protein forms a 1:1 complex with PAP1 in vitro. At 37 degrees C, a thermosensitive allele of FIP1 shows a shortening of poly(A) tails and a decrease in the steady-state level of actin transcripts. When assayed for 3'-end processing in vitro, fip1 mutant extracts exhibit normal cleavage activity, but fail to polyadenylate the upstream cleavage product. Polyadenylation activity is restored by adding polyadenylation factor I (PF I). Antibodies directed against FIP1 specifically recognize a polypeptide in these fractions. Coimmunoprecipitation experiments reveal that RNA14, a subunit of cleavage factor I (CF I), directly interacts with FIP1, but not with PAP1. We propose a model in which PF I tethers PAP1 to CF I, thereby conferring specificity to poly(A) polymerase for pre-mRNA substrates.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Preker PJ, Lingner J, Minvielle-Sebastia L, Keller W
Primary Lit For
FIP1 | mRNA cleavage factor complex CFI
Additional Lit For
ACT1 | PAP1 | RNA14 | fip1-1

Gene Ontology Annotations 4 entries for 1 gene


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene/ComplexQualifierGene Ontology TermAnnotation ExtensionEvidenceSourceAssigned On
FIP1enablesprotein-macromolecule adaptor activityhas input PAP1IPI with PAP1SGD2013-08-07
FIP1enablesprotein-macromolecule adaptor activityhas input RNA14IPI with PAP1SGD2013-08-07
FIP1enablesprotein-macromolecule adaptor activityhas input PAP1IPI with RNA14SGD2013-08-07
FIP1enablesprotein-macromolecule adaptor activityhas input RNA14IPI with RNA14SGD2013-08-07
Showing 1 to 4 of 4 entries

Interaction Annotations


Genetic Interactions

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

Physical Interactions 1 entry for 2 genes

Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

InteractorInteractorAssayAnnotationActionModification
FIP1RNA14Affinity Capture-Westernmanually curatedBait-HitNo Modification
Showing 1 to 1 of 1 entries