The main complex formed in solution between lysine:tRNA ligase from yeast and tRNA is composed of two enzyme molecules and one molecule of tRNA as shown by an equilibrium analysis of small angle X-ray scattering data recorded at 21 deg C in the pH range 6.6 to 7.4. The stability of the complex with both cognate and non-cognate tRNA decreases with increasing Mg 2+ concentration. The two types of complexes differ, however, in as much as the stability of the non-cognate complex is independent of pH while the stability of the cognate complex decreases with increasing pH. Analysis of the small-angle X-ray scattering from the complex between enzyme and cognate tRNA gave a radius of gyration of 57.4 A, a molecular weight of 236,000 and a volume of 600,000 A(3), in agreement with the theoretical values calculated for a complex containing two enzyme molecules and one tRNA (molecular weight 252,600 and volume 631,000 A(3). A comparison of the experimental data with theoretical scattering curves computed for different triaxial bodies suggests that the two ellipsoid protein molecules interact with their longest semi-axes at right angles to each other, and that within the angle formed, partly engulfed by the protein ellipsoids, is the supposedly L-shaped tRNA molecule.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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