Reference: Koettnitz K, et al. (1995)
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Abstract
Using an oligodeoxynucleotide generated by rapid PCR amplification of 5'-cDNA ends (5'-RACE) as a detection probe, we have isolated a new genomic clone encoding the human eukaryotic initiation factor 5A (eIF-5A). Sequence analysis revealed that the eIF-5A coding region is identical to the corresponding cDNA but interrupted by three introns. In a plasmid shuffle experiment we show functional replacement of the essential homologous gene in Saccharomyces cerevisiae by this human eIF-5A.
- Reference Type
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Journal Article
- Authors
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Koettnitz K,
Wöhl T,
Kappel B,
Lottspeich F,
Hauber J,
Bevec D
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