Reference: Kurzweilová H and Sigler K (1993) Fluorescent staining with bromocresol purple: a rapid method for determining yeast cell dead count developed as an assay of killer toxin activity. Yeast 9(11):1207-11

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Abstract


A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5.2. The method was used to determine the activity of Saccharomyces cerevisiae pore-forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test.

Reference Type
Comparative Study | Evaluation Study | Journal Article | Research Support, Non-U.S. Gov't
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Kurzweilová H, Sigler K
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