We have studied in lethal and mutagenic effects and the nature of mutations induced by 3H decays in the 5-th position of cytosine (5-3H-C). The lethal efficiency was determined as alpha 1 = (10.3 +/- 6.7) x 10(-3) decay-1 or alpha 1 = (12.9 +/- 9.4) x 10(-5) rad-1 and the mutagen efficiency for ade1, ade2 genes -- as alpha m = (4.3 +/- 2.3) x 10(-7) decay-1 or alpha m = (5.4 +/- 2.9) x 10(-9) rad-1. For ade2 gene the spectrum of mutations induced by 5-3H-C was as follows: 1% of frameshifts and 99% of base pair substitutions -- 9% of transversions, 3% of AT leads to GC transitions and 87% of GC leads to AT transitions. Our results establish the 5-3H-C as one of the most effective and specific mutagens reported so far for yeast. According to the scheme of Krasin with coworkers, the final product of 3H decay in the 5-th position of cytosine is uracil. Our calculations show that more than 90% of uracil residues are removed from the yeast genome by cell repair systems.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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