Reference: Rivin CJ and Fangman WL (1980) Replication fork rate and origin activation during the S phase of Saccharomyces cerevisiae. J Cell Biol 85(1):108-15

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Abstract


When the growth rate of the yeast Saccharomyces cerevisiae is limited with various nitrogen sources, the duration of the S phase is proportional to cell cycle length over a fourfold range of growth rates (C.J. Rivin and W. L. Fangman, 1980, J. Cell Biol. 85:96-107). Molecular parameters of the S phases of these cells were examined by DNA fiber autoradiography. Changes in replication fork rate account completely for the changes in S-phase duration. No changes in origin-to-origin distances were detected. In addition, it was found that while most adjacent replication origins are activated within a few minutes of each other, new activations occur throughout the S phase.

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Journal Article | Research Support, U.S. Gov't, P.H.S.
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Rivin CJ, Fangman WL
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