Reference: Simon M and Faye G (1984) Organization and processing of the mitochondrial oxi3/oli2 multigenic transcript in yeast. Mol Gen Genet 196(2):266-74

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Abstract


In the present article, we confirm our previous proposal (Faye and Simon 1983a, b) that the oxi3 and oli2 genes belong to the same transcription unit. Furthermore, we have shown that a primary polycistronic transcript covers oxi3, aap1, oli2 and extends beyond URF2. Transcriptional analysis of this region revealed several cleavage points. The examination of the DNA sequence at and surrounding these cleavage points disclosed that some of them take place at or near specific sequences found also in other known multigenic transcripts. Two of the major cleavages involve the stem-loop structure of GC rich clusters. We discuss the possibility that some of these cleavage sites serve as post-transcriptional processing signals and may be necessary for the maturation of the precursor RNA.

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Journal Article | Research Support, Non-U.S. Gov't
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Simon M, Faye G
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