Respiratory deficient (rho o) strains respond to liquid holding in buffer alone with a sharp decrease of cell survival. In buffer with 0.02% glucose rho o strains behave similarly as rho+ strains. After UV or DEB inactivation rho o strains derived from rho+ rad mutants capable of LHR in buffer alone, manifest this capacity in glucose buffer. In some respiratory proficient rad mutants LHR is enhanced or induced by holding in glucose buffer, e.g. DEB inactivated rad3, rad6 and rad14. In such mutants the number of mitochondrial profiles was found to be 3 to 4 times lower than in the wild-type RAD or in rad mutants with LHR insensitive to glucose. This suggests that mutants with glucose-enhanced LHR are defective in energy metabolism and require exogenous energy supply for initiation of liquid holding repair capacity. In UV-irradiated JG-1 (rad1-1), rad3 and rad11 as well as in DEB-inactivated rad7, rad11, rad19 and rad20 the inability of LHR is a constitutive phenomenon and cannot be overcome by exogenous energy supply.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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