Two mutants of Saccharomyces cereoisiae resistant to 5 x 10^-3 M thiaisoleucine were found to have threonine deaminases 10- and 100-fold less sensitive than the wild type to L-isoleucine as inhibitor. The apparent affinity for L-threonine as substrate, L-valine as activator, and L-isoleucine as activator was unchanged. Reversal of the isoleucine inhibition required higher concentrations of valine in the mutants as compared to the wild type. The enzyme was shown to exhibit cooperativity with respect to substrate in the absence of effecors, and with respect to activators as well. The kinetics of activation by valine or by isoleucine may be described by a modification of the allosteric equation of Monod, Wyman, and Changeux (1965, J. Mol. Biol. 12, 88). A tentative model for the interactions of the enzyme with its substrate and effectors, with two sorts of sites able to bind isoleucine or valine, is proposed. This model accounts for the activation as well as the inhibition by isoleucine.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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