This study reports an enhancement of ethanol yield in Saccharomyces cerevisiae with low-intensity ultrasonic irradiation using fixed mode frequency generated by a self-developed six-frequencies (20, 23, 25, 28, 33, 40 kHz) ultrasonic device in our group. After sonication treatment, the ethanol production potential was determined. Under the optimal conditions of ultrasonic treatment (ultrasonic frequency 28 kHz, power density 180 W/L, and treatment time 24 h), the maximum ethanol yield increased by 34.87% compared to the control. Transcriptome sequencing showed that the ultrasonic treatment had expressional regulations on genes involved in pyruvate metabolism, glycolysis, pentose phosphate pathway, glucose transport, and reducing power production. The quantitative real-time polymerase chain reaction (qRT-PCR) further confirmed the changes in gene expression (up- or down-regulation). Metabolomics revealed that ultrasonic treatments increased intracellular glucose and nicotinamide adenine dinucleotide (NADH) contents, which are key metabolites for ethanol synthesis. Besides, ultrasonic treatments decreased the acetate and its derivatives resulting in lowered reverse consumption of pyruvate and thus promoted ethanol synthesis. These changes in gene expression and metabolites content might be the main reason why the ethanol yield in Saccharomyces cerevisiae increased after ultrasonic irradiation.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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